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"Expression profiling reveals off-target gene regulation by RNAi,"
by Aimee L. Jackson and 8 others, Nature Biotechnology, 21(6): 635-7,
June 2003.
[Authors' affiliation: Rosetta Inpharmatics, Kirkland, WA]
Abstract:
"RNA interference is thought to require near-identity between the small
interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression
profiling to characterize the specificity of gene silencing by siRNAs in
cultured human cells. Transcript profiles revealed siRNA-specific rather than
target-specific signatures, including direct silencing of nontargeted genes
containing as few as eleven contiguous nucleotides of identity to the siRNA.
These results demonstrate that siRNAs may cross-react with targets of limited
sequence similarity."
This 2003 report in Nature Biotechnology
was cited 40 times in current journal articles indexed by
Thomson
Scientific during March-April 2005. At present, with its latest two-month
total, this paper ranks at #4 among
non-review papers in biology published in the last two years. Prior to the
most recent bimonthly count, citations
to the paper have accrued as follows:
January-February 2005: 29 citations
November-December 2004: 23
September-October 2004: 27
July-August 2004: 24
May-June 2004: 13
March-April 2004: 22
January-February 2004: 12
November-December 2003: 12
September-October 2003: 12
July-August 2003: 1
Total citations to date: 215
SOURCE: Hot
Papers Database (Included with a subscription to the ISI print newsletter Science
Watch®, available from the ISI
Research Services Group. Packaged on a CD-ROM that is mailed with each Science
Watch issue, the Hot
Papers Database contains data on hundreds of highly cited papers published
during the last two years. User interface permits searching by author,
organization, journal, field, and more. Total citations, as well as citations
accrued during successive bimonthly periods, can be assessed and graphed. An
updated CD containing the most recent bimonthly data is mailed with every new
issue of Science
Watch,
six times a year. The CD also includes an electronic version of the Science
Watch
issue in HTML format, for personal desktop access.)
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