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"A system for stable expression of
short interfering RNAs in mammalian cells,"
by Thijn R. Brummelkamp,
Rene Bernards, and Reuven Agami, Science, 296[5567]: 550-3, 19 April
2002.
[Authors' affiliations: Netherlands Cancer
Institute, Amsterdam; Center for Biomedical Genetics, Amsterdam]
Abstract: "Mammalian genetic
approaches to study gene function have been hampered by the lack of tools to
generate stable loss-of-function phenotypes efficiently. We report here a new
vector system, named pSUPER, which directs the synthesis of small interfering
RNAs (siRNAs) in mammalian cells. We show that siRNA expression mediated by
this vector causes efficient and specific down-regulation of gene expression,
resulting in functional inactivation of the targeted genes. Stable expression
of siRNAs using this vector mediates persistent suppression of gene
expression, allowing the analysis of loss-of-function phenotypes that develop
over longer periods of time. Therefore, the pSUPER vector constitutes a new
and powerful system to analyze gene function in a variety of mammalian cell
types."
This 2002 report from Science was
cited 72 times in current journal articles indexed by Thomson
ISI during March-April 2004. During that two-month interval this was the
second-most-cited paper in biology published in the last two years, aside from
reviews. Prior to the most recent bimonthly period, citations to the paper
have accrued as follows:
January-February 2004: 50 citations
November-December 2003: 47
September-October 2003: 60
July-August 2003: 44
May-June 2003: 28
March-April 2003: 32
January-February 2003: 19
November-December 2002: 24
September-October 2002: 13
July-August 2002: 7
May-June 2002: 2
Total citations to date: 398
Related information:
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February 29, 2004
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