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"Systematic identification of protein
complexes in Saccharomyces cerevisiae by mass spectrometry,"
by
Yuen Ho and 45 others, Nature, 415(6868): 180-3, 10 January 2002.
[Authors' affiliations: MDS Proteomics,
Odense, Denmark; Mount Sinai Hospital, Toronto, Canada; University of
Toronto, Canada]
Abstract: "The recent abundance of genome sequence data has
brought an urgent need for systematic proteomics to decipher the encoded
protein networks that dictate cellular function. To date, generation of
large-scale protein-protein interaction maps has relied on the yeast
two-hybrid system, which detects binary interactions through activation of
reporter gene expression. With the advent of ultrasensitive mass spectrometric
protein identification methods, it is feasible to identify directly protein
complexes on a proteome-wide scale. Here we report, using the budding yeast Saccharomyces
cerevisiae as a test case, an example of this approach, which we term
high-throughput mass spectrometric protein complex identification (HMS-PCI).
Beginning with 10% of predicted yeast proteins as baits, we detected 3,617
associated proteins covering 25% of the yeast proteome. Numerous protein
complexes were identified, including many new interactions in various
signalling pathways and in the DNA damage response. Comparison of the HMS-PCI
data set with interactions reported in the literature revealed an average
threefold higher success rate in detection of known complexes compared with
large-scale two-hybrid
studies. Given the high degree of connectivity observed in this study, even
partial HMS-PCI coverage of complex proteomes, including that of humans,
should allow comprehensive identification of cellular networks."
This 2002 report from Nature was cited
35 times in current journal articles indexed by Thomson ISI
during
May-June 2003. As was the case in the previous count for March-April, the
latest two-month total makes this
the third-most-cited paper in all of biology published in the last two years,
save for reviews. Prior to the most
recent bimonthly count, citations to the paper have accrued as follows:
March-April 2003: 69 citations
January-February 2003: 47
November-December 2002: 32
September-October 2002: 21
July-August 2002: 21
May-June 2002: 14
March-April 2002: 7
January-February 2002: 3
Total citations to date: 249
SOURCE: Hot
Papers Database (Included with a subscription to the ISI print newsletter Science
Watch®, available from the ISI
Research Services Group. Packaged on a CD-ROM that is mailed with each Science
Watch issue, the Hot
Papers Database contains data on hundreds of highly cited papers published
during the last two years. User interface permits searching by author,
organization, journal, field, and more. Total citations, as well as citations
accrued during successive bimonthly periods, can be assessed and graphed. An
updated CD containing the most recent bimonthly data is mailed with every new
issue of Science
Watch,
six times a year. The CD also includes an electronic version of the Science
Watch
issue in HTML format, for personal desktop access.)
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