|
"Functional organization of the yeast
proteome by systematic analysis of protein complexes,"
by Anne-Claude Gavin and 37 others, Nature, 415(6868): 141-7, 10 January 2002.
[Authors' affiliations: Cellzome AG,
Heidelberg, Germany; EMBL, Heidelberg, Germany; CGM-CNRS,
Gif sur Yvette, France]
Abstract: "Most cellular
processes are carried out by multiprotein complexes. The identification and
analysis of their
components provides insight into how the ensemble of expressed proteins
(proteome) is organized into functional units. We used tandem-affinity purification (TAP) and mass spectrometry in a
large-scale approach to characterize multiprotein complexes in Saccharomyces
cerevisiae. We processed 1,739 genes, including 1,143 human orthologues of
relevance to human biology, and purified 589 protein assemblies. Bioinformatic analysis
of these assemblies defined 232 distinct multiprotein complexes and proposed new cellular roles for 344 proteins,
including 231 proteins with no previous functional annotation. Comparison of
yeast and human complexes showed that conservation across species extends from
single proteins to their molecular environment. Our analysis provides an outline of
the eukaryotic proteome as a network of protein complexes at a level of organization beyond binary interactions. This
higher-order map contains fundamental biological information and offers the context for a more reasoned and informed
approach to drug discovery."
This report from early 2002 in Nature was cited 51 times
in current journal articles indexed by Thomson ISI
during January-February 2003. Only two other biology papers published in the
last two years, aside from
reviews, collected a greater number of citations during that two-month period.
Prior to the most recent bimonthly
count, citations to the paper have accrued as follows:
November-December 2002: 44 citations
September-October 2002: 26
July-August 2002: 24
May-June 2002: 21
March-April 2002: 10
January-February 2002: 3
Total citations to date: 179
SOURCE: Hot
Papers Database (Included with a subscription to the ISI print newsletter Science
Watch®, available from the ISI
Research Services Group. Packaged on a CD-ROM that is mailed with each Science
Watch issue, the Hot
Papers Database contains data on hundreds of highly cited papers published
during the last two years. User interface permits searching by author,
organization, journal, field, and more. Total citations, as well as citations
accrued during successive bimonthly periods, can be assessed and graphed. An
updated CD containing the most recent bimonthly data is mailed with every new
issue of Science
Watch,
six times a year. The CD also includes an electronic version of the Science
Watch
issue in HTML format, for personal desktop access.)
Previous Page | Return to SCI-BYTES
Main Menu
| Return to 2003 Menu
If you came from the Thomson Scientific Web site, click
here to return
|
cites
:
