|
"Duplexes of 21-nucleotide RNAs
mediate RNA interference in cultured mammalian cells,"
by Sayda M. Elbashir, Jens Harborth, Winfried Lendeckel, Abdullah Yalcin,
Klaus Weber, and Thomas Tuschl, Nature, 411(6836): 494-8, 24 May 2001.
[Authors' affiliation: Max Planck Institute
for Biophysical Chemistry, Gottingen, Germany]
Abstract: "RNA interference (RNAi)
is the process of sequence-specific, post-transcriptional gene silencing in
animals and plants, initiated by double-stranded RNA (dsRNA) that is
homologous in sequence to the silenced gene. The mediators of
sequence-specific messenger RNA degradation are 21- and 22-nucleotide small
interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer
dsRNAs. Here we show that 21-nucleotide siRNA duplexes specifically suppress
expression of endogenous and heterologous genes in different mammalian cell
lines, including human embryonic kidney and HeLa cells. Therefore,
21-nucleotide siRNA duplexes provide a new tool for studying gene function in
mammalian cells and may eventually be used as gene-specific
therapeutics."
This 2001 report from Nature was cited
75 times in current journal articles indexed by Thomson ISI
during
November-December 2002. Only two other biology papers published in the last
two years (aside from reviews)
attracted a greater number of citations during that two-month period. Prior to
the most recent bimonthly count,
citations to the paper have accrued as follows:
September-October 2002: 54 citations
July-August 2002: 40
May-June 2002: 46
March-April 2002: 33
January-February 2002: 22
November-December 2001: 19
September-October 2001: 9
July-August 2001: 5
May-June 2001: 1
Total citations to date: 304
SOURCE: Hot
Papers Database (Included with a subscription to the ISI print newsletter Science
Watch®, available from the ISI
Research Services Group. Packaged on a CD-ROM that is mailed with each Science
Watch issue, the Hot
Papers Database contains data on hundreds of highly cited papers published
during the last two years. User interface permits searching by author,
organization, journal, field, and more. Total citations, as well as citations
accrued during successive bimonthly periods, can be assessed and graphed. An
updated CD containing the most recent bimonthly data is mailed with every new
issue of Science
Watch,
six times a year. The CD also includes an electronic version of the Science
Watch
issue in HTML format, for personal desktop access.)
Previous Page | Return to SCI-BYTES
Main Menu
| Return to 2003 Menu
If you came from the Thomson Scientific Web site, click
here to return
|
cites
:
