|
"Duplexes of 21-nucleotide RNAs
mediate RNA interference in cultured mammalian cells,"
by Sayda M.
Elbashir, Jens Harborth, Winfried Lendeckel, Abdullah Yalcin, Klaus Weber, and
Thomas Tuschl, Nature,
411(6836): 494-8, 24 May 2001.
[Authors' affiliation: Max Planck Institute
for Biophysical Chemistry, Gottingen, Germany]
Abstract: "RNA interference (RNAi)
is the process of sequence-specific, post-transcriptional gene silencing
in animals and plants, initiated by double-stranded RNA (dsRNA) that is
homologous in sequence to the silenced
gene. The mediators of sequence-specific messenger RNA degradation are 21- and
22-nucleotide small interfering
RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Here
we show that 21-nucleotide
siRNA duplexes specifically suppress expression of endogenous and heterologous
genes in different mammalian
cell lines, including human embryonic kidney and HeLa cells. Therefore,
21-nucleotide siRNA duplexes provide
a new tool for studying gene function in mammalian cells and may eventually be
used as gene-specific therapeutics."
This 2001 report from Nature was cited
67 times in current journal articles indexed by Thomson ISI
during
January-February 2003. That two-month citation tally made this the
second-most-cited paper in biology
(aside from reviews) published in the last two years. Prior to the most recent
bimonthly count, citations to
the paper have accrued as follows:
November-December 2002: 75 citations
September-October 2002: 54
July-August 2002: 40
May-June 2002: 46
March-April 2002: 33
January-February 2002: 22
November-December 2001: 19
September-October 2001: 9
July-August 2001: 5
May-June 2001: 1
Total citations to date: 371
SOURCE: Hot
Papers Database (Included with a subscription to the ISI print newsletter Science
Watch®, available from the ISI
Research Services Group. Packaged on a CD-ROM that is mailed with each Science
Watch issue, the Hot
Papers Database contains data on hundreds of highly cited papers published
during the last two years. User interface permits searching by author,
organization, journal, field, and more. Total citations, as well as citations
accrued during successive bimonthly periods, can be assessed and graphed. An
updated CD containing the most recent bimonthly data is mailed with every new
issue of Science
Watch,
six times a year. The CD also includes an electronic version of the Science
Watch
issue in HTML format, for personal desktop access.)
Previous Page | Return to SCI-BYTES
Main Menu
| Return to 2003 Menu
If you came from the Thomson Scientific Web site, click
here to return
|
cites
:
